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In the maturation process, Langerhans cells undergo various phenotypical and functional modifications including: (i) reduction in phagocytic ability, (ii) upregulation of the expression of co-stimulatory cell surface molecules, such as CD86, CD83, CD54, CD40, and the major histocompatibility complex class II (MHC II) molecules, (iii) changes in chemokine receptor profile-downregulation of skin-homing chemokine receptors (CCR1, CCR2, CCR5, and CCR6), and (iv) upregulation of chemokine receptors taking part in the migration (CCR4, CXCR4, and CCR7) [37,38,39,40]

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If a setup includes recirculation lines, awkward placement, or access problems, it can also create added maintenance headaches